Bioremediation Potential of Fungi Isolates on Copper

Hoh, Xin Han (2016) Bioremediation Potential of Fungi Isolates on Copper. Other thesis, INTI International University.

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Abstract

Nowadays, heavy metal pollution had endangered not only living organism, but also health of natural environment. This phenomenon is usually caused by anthropogenic activities, for example heavy metal discharge to water source and mining, which causes damages to air, water as well as land areas. However, this problem is treatable either by biological or chemical approaches. One of the most common and cost-effective of biological method to treat the contamination sites is bioremediation. In this research paper, fungi isolates were used to conduct the study, namely nycoremediation. The aim of this study is to study the potential of Aspergillus niger, Aspergillus tamari, Simplicillium subtropicum and Fusarium solani in bioremediating copper metal, and hence determine their bioremediation efficiency. From the stock culture of the four fungi, they were inoculated to fresh potato dextrose agar (PDA) plate to obtain pure culture. With copper concentration range from 0ppm (control) to 200ppm, pure culture is inoculated into solution containing potato dextrose broth and copper sulphate (II) salt to allow growth and metal uptake. Triplicates of medium were done to provide consistent results. After cultivation period of 7-10 days, biomasses were harvested and homogenized. The biomasses were sent to inductively-coupled plasma mass spectrometry (ICP-MS) to determine metal uptake rate after measuring the dry weight of fungi biomasses. The results obtained showed S. subtropicum grew best, with 0.806g dry biomass weight, at 50 ppm of CuSO4. Whereas for result ICP-MS, S. subtropicum showed outstanding biosorption capacity among other isolates. After analysis of the results obtained, it can concluded that Simplicillium subtropicum was the best isolates in bioremediation of copper with respect to ICP-MS result and growth rate measured by mass

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