Nur Nadiah, Zainal Ariff (2016) Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production. Other thesis, INTI International University.
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Abstract
Four bacterial isolates that were known to degrade hydrocarbons were screened for copper (Cu) resistance and biosurfactant production. Based on spectrophotometry, isolate D had the highest growth (p<0.05) followed by isolate A, isolate C and isolate B with the least growth. Biosurfactant screening assays confirmed that both isolates D and A produced biosurfactant. The dry-weight of biosurfactant obtained from partial purification suggested that there was a direct relationship between the number of cells present and the amount of biosurfactant produced. Gram staining showed that isolate D and isolate A are gram negative and gram positive respectively. The results showed that gram negative bacteria was able to show tolerance towards 20.0 mg/L Cu, possible by secretion of biosurfactant to chelate Cu ions in reducing the metal toxicity.
| Item Type: | Thesis (Other) |
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| Additional Information: | BBTEI 115 |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Faculty of Health & Life Sciences |
| Depositing User: | Unnamed user with email masilah.mansor@newinti.edu.my |
| Date Deposited: | 24 Aug 2017 08:43 |
| Last Modified: | 24 Aug 2017 08:43 |
| URI: | http://eprints.intimal.edu.my/id/eprint/891 |
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