Ainon, Hamzah and Wong, Kok Kee (2015) Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation. Annals of Microbiology, 65 (2). pp. 1131-1136. ISSN 1869-2044
Full text not available from this repository.Abstract
Four methyl tert-butyl ether (MTBE)-degrading bacteria were isolated from a processing plant in Malaysia. Based on 16S rDNA sequences, the four isolates were identified as Exiguobacterium profundum P1M-2, Bacillus megaterium P1M-11, Alishewanella sp. P2A-12 and Pseudomonas mendocina P2M-8. Each of the isolates obtained optimum growth using a different source of nitrogen (0.1- 0.03 % yeast or peptone) and all four isolates were able to biodegrade 92.05-99.98 % of MTBE within seven days. Amongst the four isolates, the highest percentage of MTBE degradation was achieved using B. megaterium P1M-11. The highest growth on tert-butyl alcohol (TBA), tert-amyl alcohol (TAA) and 2-hydroxyisobutyric acid (2-HIBA) was also observed in B. megaterium P1M-11. This study suggests MTBE degradation by each of the bacteria can be enhanced by choosing the right nitrogen source. Furthermore, the ability of B. megaterium P1M-11 to grow on primary metabolites of MTBE and other structurally related ethers suggests the secretion of diverse degradative enzymes, making this isolate a good candidate to be applied in MTBE bioremediation strategies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Degradation, Exiguobacterium profundum, Bacillus megaterium, Alishewanella, Pseudomonas mendocina, MTBE, Nitrogen source |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biotechnology |
| Depositing User: | Unnamed user with email masilah.mansor@newinti.edu.my |
| Date Deposited: | 25 Oct 2016 06:25 |
| Last Modified: | 25 Oct 2016 06:26 |
| URI: | http://eprints.intimal.edu.my/id/eprint/534 |
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