The Effects of Clinacanthus nutans Leaf Extract Towards Tyrosinase and Collagenase Activities

Lim, She Yan (2017) The Effects of Clinacanthus nutans Leaf Extract Towards Tyrosinase and Collagenase Activities. Other thesis, INTI INTERNATIONAL UNIVERSITY.

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Abstract

Clinacanthus nutans is a plant popular among Southeast Asia countries as a traditional medicine to treat various diseases. C. nutans is believed to have potential of growth in different industries due to its anti-diabetic, anti-viral and anti-cancer activities. The undesired formation of black or brown pigmentation on human skin involves tyrosinase while the breakdown of collagen in dermis of skin cell involves collagenase. The effects caused by these two enzymes indicate the aging of skin cells, hence this problem remains as a hot issue. To date, there are not much research done on the anti-tyrosinase and anti-collagenase potential of C. nutans. Thus, this study aimed to quantify total flavonoid content (TFC) found in C. nutans crude leaf extract and to determine the tyrosinase and collagenase activities of C. nutans crude leaf extract. Aluminium chloride colorimetric technique was performed to quantify TFC. Inhibition % of various compounds such as C. nutans crude leaf extract, kojic acid and ascorbic acid toward tyrosinase was calculated. The binding affinity of tyrosinase was determined by using both tyrosine and C. nutans crude leaf extract whereas the binding affinity of collagenase was examined by using FALGPA and also C. nutans crude leaf extract. Their Km values were identified from the same Lineweaver-Burk plot. C. nutans crude leaf extract contained 28.70 ± 0.93 mg QUE per gram of fresh leaf. The % inhibition of tyrosinase of C. nutans crude leaf extract, ascorbic acid and kojic were 15.11%, 84.46% and 87.01% respectively. For tyrosinase activity, the Km value of tyrosine and C. nutans crude leaf extract was 0.833 mg/mL and 2.000 mg/mL respectively. Both compounds have the same Vmax value of 1.7 × 10-2 (mg/mL).min-1. For collagenase activity, the Km values of FALGPA and C. nutans crude leaf extract was 0.083 mg/mL and 0.330 mg/mL respectively. Both compounds have the same Vmax value of 2.78 × 10-3(mg/mL).min-1 as well. In conclusion, C. nutans crude leaf extract has low % inhibition toward tyrosinase, high Km value toward tyrosinase and collagenase might suggest lack of its certain structural group towards both enzymes.

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