Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production

Nur Nadiah, Zainal Ariff (2016) Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production. Other thesis, INTI International University.


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Four bacterial isolates that were known to degrade hydrocarbons were screened for copper (Cu) resistance and biosurfactant production. Based on spectrophotometry, isolate D had the highest growth (p<0.05) followed by isolate A, isolate C and isolate B with the least growth. Biosurfactant screening assays confirmed that both isolates D and A produced biosurfactant. The dry-weight of biosurfactant obtained from partial purification suggested that there was a direct relationship between the number of cells present and the amount of biosurfactant produced. Gram staining showed that isolate D and isolate A are gram negative and gram positive respectively. The results showed that gram negative bacteria was able to show tolerance towards 20.0 mg/L Cu, possible by secretion of biosurfactant to chelate Cu ions in reducing the metal toxicity.

Item Type: Thesis (Other)
Additional Information: BBTEI 115
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Health & Life Sciences
Depositing User: Unnamed user with email
Date Deposited: 24 Aug 2017 08:43
Last Modified: 24 Aug 2017 08:43

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