Anti-Oxidant Content And Inhibitory Effects of Leaf Extract of Piper sarmentosum Toward Collagenase

Chin, Kai Shin (2017) Anti-Oxidant Content And Inhibitory Effects of Leaf Extract of Piper sarmentosum Toward Collagenase. Other thesis, INTI INTERNATIONAL UNIVERSITY.

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Abstract

Piper sarmentosum leaves possesses various types of phytochemical activity that yet to be discovered. This study aims to determine the total flavonoid content (TFC), radical scavenging and collagenase activity in crude leafextract of P. sarmentosum. Aluminum chloride colorimetric method was employed to determineradical scavenging activity between crude leaf extract, QU standard and ascorbic acid. The binding affinity of collagenase was determined using various compounds (EDTA, QU standard, crude leaf extract and gelatin) and their Km was determined from Lineweaver-Burk plot. TFC. TFC in crude leaf extract was measured as 52.0 ± 0.79mg of QUE per gram of tissue whereas QU contents in crude leaf extract found to possess 7.0 ± 0.58 mg QUE per gram of tissues. Ascorbic acid had significantly highest radical scavenging activity as 89.4% compared to QU standard (65.3%) and crude leaf extract (49.4%). QU standard, crude leaf extract, gelatin and EDTA were ranked from lower to high according to their Km as 0.14 mM. 0.50 mM and 2.00 mM. The binding affinity of each compound was directly proportional with to Km. Crude leaf bind to the active site of collagenase by competing with gelatin. Crude leaf extract and gelatin have no significant different in term of binding affinity randomly to the active site collagenase. The study revealed that cude leaf extract may act as potential anti-aging source by scavenging radicals and inhibiting collagenase.

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